3M2T

The crystal structure of dehydrogenase from Chromobacterium violaceum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	110
Detector technology	CCD
Collection date	2010-02-26
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 21 21 2
Unit cell lengths	138.860, 63.386, 103.629
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.814 - 2.300
R-factor	0.185
Rwork	0.183
R-free	0.23050
Structure solution method	SAD
RMSD bond length	0.016
RMSD bond angle	1.635
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.050	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.107	0.538
Number of reflections	41479
<I/σ(I)>	19.8	5.2
Completeness [%]	100.0	100
Redundancy	14.2	14.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.1 M Sodium chloride, 0.1 M Bis-Tris pH 6.5, 1.5 M Ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 291K