3M2P
The crystal structure of UDP-N-acetylglucosamine 4-epimerase from Bacillus cereus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2009-02-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 104.116, 149.725, 166.326 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.701 - 2.950 |
| R-factor | 0.2149 |
| Rwork | 0.212 |
| R-free | 0.27570 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.055 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 111.280 | 3.110 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.145 | 0.475 |
| Number of reflections | 55533 | |
| <I/σ(I)> | 13.5 | 5.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.3 | 14.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 2.4 M Sodium malonate pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
