3M1C

Crystal structure of the conserved herpesvirus fusion regulator complex gH-gL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2009-03-05
Detector	ADSC QUANTUM 315
Spacegroup name	P 41 21 2
Unit cell lengths	88.255, 88.255, 333.406
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.200 - 3.000
R-factor	0.176
Rwork	0.170
R-free	0.24200
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.215
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	PHENIX (1.4_175)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.890
High resolution limit [Å]	2.840	2.840
Rmerge	0.133
Number of reflections	32326
<I/σ(I)>	5.6
Completeness [%]	99.8	100
Redundancy	5.9	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1			298	20% PEG 4000, 0.1M Sodium citrate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K