3M16
Structure of a Transaldolase from Oleispira antarctica
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-27 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97942, 0.97972 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 143.105, 143.105, 119.724 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.780 - 2.790 |
| R-factor | 0.21114 |
| Rwork | 0.209 |
| R-free | 0.24886 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1onr |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.468 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.780 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.088 | 0.734 |
| Number of reflections | 18469 | |
| <I/σ(I)> | 57.4 | 4.9 |
| Completeness [%] | 99.9 | 98.9 |
| Redundancy | 26.2 | 25.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | 4M sodium formate, 3mM magnesium chloride, cryoprotected in Paratone-N oil (Hampton), VAPOR DIFFUSION, HANGING DROP, temperature 294K, pH 8.5 |
