3LZT

REFINEMENT OF TRICLINIC LYSOZYME AT ATOMIC RESOLUTION

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1994-12
Detector	MARRESEARCH
Spacegroup name	P 1
Unit cell lengths	26.650, 30.800, 33.630
Unit cell angles	88.30, 107.40, 112.20

Refinement procedure

Resolution	20.000 - 0.925
R-factor	0.093
R-free	0.11360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.021
RMSD bond angle	6.840 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXL-96
Refinement software	SHELXL-96

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	0.940
High resolution limit [Å]	0.925	0.925
Rmerge	0.028	0.170
Total number of observations	232156 *
Number of reflections	58373
<I/σ(I)>	29.1	4.9
Completeness [%]	90.1	78
Redundancy	2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.5 *	296 *	BATCH METHOD USED. 1% PROTEIN SOLUTION IN 100MM SODIUM ACETATE PH 4.5-4.6. SODIUM NITRATE ADDED TO A CONCENTRATION OF 20MGS/ML. CRYSTALS GROWN AT ROOM TEMPERATURE., batch method

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein solution	1 (%)
2	1	1	sodium acetate	100 (mM)
3	1	2	sodium nitrate	2 (%(w/v))