3LZL

Crystal Structure Analysis of the as-solated P19 protein from Campylobacter jejuni at 1.45 A at pH 9.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-05
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.127
Spacegroup name	P 21 21 2
Unit cell lengths	54.533, 73.545, 75.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.770 - 1.450
R-factor	0.14337
Rwork	0.141
R-free	0.18668
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	P6222 incomplete SeMAD model at 2.8 A resolution.
RMSD bond length	0.017
RMSD bond angle	1.531
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	REFMAC
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.210	1.530
High resolution limit [Å]	1.450	1.450
Rmerge	0.040	0.517
Number of reflections	53633
<I/σ(I)>	14.8	2.3
Completeness [%]	98.6	97.3
Redundancy	4.4	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	298	50% POLYETHYLENE GLYCOL (PEG) 250, 0.1 M CHES (2-(N-CYCLOHEXYLAMINO) ETHANE SULFONIC ACID) BUFFER pH 9.0, CRYOGENIC CONDITION 30% GLYCEROL IN PRECIPITANT MIXTURE, VAPOR DIFFUSION, HANGING DROP, temperature 298K