3LZ0

Crystal Structure of Nucleosome Core Particle Composed of the Widom 601 DNA Sequence (orientation 1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	90
Detector technology	PIXEL
Collection date	2009-12-15
Detector	PSI PILATUS 6M
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	107.370, 109.660, 175.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	93.040 - 2.500
R-factor	0.269
Rwork	0.268
R-free	0.31800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	NCP146b (pdb code 1KX4)
RMSD bond length	0.009
RMSD bond angle	1.453
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.15)
Phasing software	PHASES
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	93.040	107.370	2.640
High resolution limit [Å]	2.500	7.910	2.500
Rmerge		0.065	0.384
Total number of observations		12042	13579
Number of reflections	65509
<I/σ(I)>	7.8	5.1	1.9
Completeness [%]	90.6	98.9	61.9
Redundancy	4.6	4.9	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	K cacodylate, KCl, MnCl2, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K