3LYP
Structure of stringent starvation protein A homolog from Pseudomonas fluorescens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.591, 53.917, 54.496 |
| Unit cell angles | 85.28, 71.05, 66.71 |
Refinement procedure
| Resolution | 50.000 - 1.600 |
| R-factor | 0.185 |
| Rwork | 0.183 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yy7 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.528 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 4.340 | 1.600 |
| Rmerge | 0.073 | 0.071 | 0.201 |
| Number of reflections | 54196 | ||
| <I/σ(I)> | 17.7 | ||
| Completeness [%] | 95.4 | 86.5 | 94 |
| Redundancy | 3.2 | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 0.1 M Imidazole pH 8.0, 0.4M Sodium Phosphate, 1.6M Potassium Phosphate, 0.2M Nacl, Vapor diffusion, Sitting drop, temperature 298K |
