3LY7
Crystal structure of the periplasmic domain of CadC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-28 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 83.898, 83.898, 199.090 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 72.660 - 1.800 |
| R-factor | 0.192 |
| Rwork | 0.191 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lya |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.217 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.660 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.274 | |
| Number of reflections | 39362 | |
| <I/σ(I)> | 9.5 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.3 | 15.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 1.5 M ammonium sulfate, 7% (v/v) 2-propanol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
