3LY1

Crystal structure of Putative histidinol-phosphate aminotransferase (YP_050345.1) from Erwinia carotovora atroseptica SCRI1043 at 1.80 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-07
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.96109,0.97946,0.97936
Spacegroup name	P 1 21 1
Unit cell lengths	58.625, 150.477, 100.092
Unit cell angles	90.00, 94.38, 90.00

Refinement procedure

Resolution	29.564 - 1.800
R-factor	0.178
Rwork	0.176
R-free	0.20600
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.533
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.564	29.564	1.850
High resolution limit [Å]	1.800	3.850	1.790
Rmerge	0.059	0.022	0.675
Number of reflections	158547	31348	24110
<I/σ(I)>	8.36	24.7	1.4
Completeness [%]	96.5	97	79.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	277	20.0000% iso-Propanol, 20.0000% PEG-4000, 0.1M Citrate pH 5.6, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K