3LXT
Crystal structure of Glutathione S Transferase from Pseudomonas fluorescens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9192 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.681, 57.887, 265.772 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.300 - 1.760 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.22600 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CCP4 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.820 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.070 | 0.220 |
| Number of reflections | 80688 | |
| <I/σ(I)> | 11.9 | 5 |
| Completeness [%] | 90.2 | 54.3 |
| Redundancy | 7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 278 | 0.2M MgCl2, 0.1M Tris, 25% PEG4K, Praseodymium acetate, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 278K |
