3LXO
The crystal structure of ribonuclease A in complex with thymidine-3'-monophosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 294 |
Detector technology | IMAGE PLATE |
Collection date | 2007-11-14 |
Detector | RIGAKU RAXIS II |
Wavelength(s) | 1.514 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 64.592, 64.592, 65.364 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.954 - 1.549 |
R-factor | 0.1448 |
Rwork | 0.141 |
R-free | 0.18130 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.482 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.000 | 1.610 |
High resolution limit [Å] | 1.550 | 1.550 |
Number of reflections | 23146 | |
<I/σ(I)> | 18.1 | 1.2 |
Completeness [%] | 99.0 | 91.8 |
Redundancy | 4.8 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 294 | 20% ammonium sulfate, 2M NaCl, pH 5.5., VAPOR DIFFUSION, HANGING DROP, temperature 294K |