3LX5
Crystal structure of mGMPPNP-bound NFeoB from S. thermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-06-24 |
Detector | MAR 345 mm plate |
Wavelength(s) | 1.5412 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 49.100, 82.200, 148.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.610 - 1.900 |
R-factor | 0.199 |
Rwork | 0.197 |
R-free | 0.24100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hyr |
RMSD bond length | 0.014 |
RMSD bond angle | 1.479 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0063) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.118 | 0.336 |
Number of reflections | 23847 | |
<I/σ(I)> | 14.2 | 3.5 |
Completeness [%] | 97.8 | 85.4 |
Redundancy | 5.7 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.02M Malic acid, 0.04M MES, 0.04M Tris, 25% (w/v) PEG 1500, pH 8.0, vapor diffusion, hanging drop, temperature 298K |