3LX4
Stepwise [FeFe]-hydrogenase H-cluster assembly revealed in the structure of HydA(deltaEFG)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Detector technology | CCD |
Collection date | 2008-12-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.95369 |
Spacegroup name | P 32 |
Unit cell lengths | 70.895, 70.895, 155.429 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.970 |
R-factor | 0.173 |
Rwork | 0.170 |
R-free | 0.21600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1feh |
RMSD bond length | 0.006 |
RMSD bond angle | 0.924 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.040 |
High resolution limit [Å] | 1.970 | 1.970 |
Number of reflections | 60782 | |
<I/σ(I)> | 2.4 | |
Completeness [%] | 97.9 | 97.2 |
Redundancy | 4.4 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | capillary batch diffusion | 6.5 | 298 | 25.5% PEG 8000, 0.085 M sodium cacodylate, 0.17 M sodium acetate trihydrate, 1 mM dithionite, pH 6.5, capillary batch diffusion, temperature 298.0K |