3LWX

Crystal structure of Na(+)-translocating NADH-quinone reductase subunit C (YP_001302508.1) from Parabacteroides distasonis ATCC 8503 at 1.10 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-01-25
Detector	MARMOSAIC 325 mm CCD
Spacegroup name	P 21 21 21
Unit cell lengths	37.444, 60.693, 82.758
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.739 - 1.100
R-factor	0.117
Rwork	0.116
R-free	0.13800
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.474
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.739	29.739	1.140
High resolution limit [Å]	1.100	2.370	1.100
Rmerge	0.041	0.025	0.534
Number of reflections	74185	14688	13515
<I/σ(I)>	14.69	50.9	2.2
Completeness [%]	95.2	99.1	89.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	15.0000% Glycerol, 0.1700M NaOAc, 25.5000% PEG-4000, 0.1M TRIS pH 8.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K