3LWK
Crystal structure of human Beta-crystallin A4 (CRYBA4)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9796 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 94.790, 94.790, 90.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.420 - 1.700 |
| R-factor | 0.1622 |
| Rwork | 0.161 |
| R-free | 0.18823 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oki |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.551 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.030 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.104 | 0.720 |
| Number of reflections | 22910 | |
| <I/σ(I)> | 13.6 | 2.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.8 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293.15 | 1.5M Ammonium Phosphate, 5% Glycerol, 0.1M Tris, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K |
