3LWE
The crystal structure of MPP8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 65 |
| Unit cell lengths | 50.665, 50.665, 123.538 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.880 - 2.050 |
| R-factor | 0.22 |
| Rwork | 0.217 |
| R-free | 0.28300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dm1 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.563 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.090 |
| High resolution limit [Å] | 2.050 | 5.560 | 2.050 |
| Rmerge | 0.076 | 0.036 | 0.853 |
| Number of reflections | 11280 | ||
| <I/σ(I)> | 8.4 | ||
| Completeness [%] | 99.7 | 99.2 | 99.6 |
| Redundancy | 11.3 | 11.1 | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 25%PEG400, 0.2M MgCl2, 0.1M Hepes 7.5, VAPOR DIFFUSION |
