3LW2
Mouse Plasminogen Activator Inhibitor-1 (PAI-1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2007-11-17 |
| Detector | Pilatus |
| Wavelength(s) | 1.0001 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 150.900, 62.300, 52.200 |
| Unit cell angles | 90.00, 105.50, 90.00 |
Refinement procedure
| Resolution | 39.940 - 1.930 |
| R-factor | 0.1826 |
| Rwork | 0.181 |
| R-free | 0.21832 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dvn |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.361 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.940 | 1.980 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.474 | |
| Number of reflections | 34894 | |
| <I/σ(I)> | 4.3 | |
| Completeness [%] | 97.0 | 95.5 |
| Redundancy | 6.4 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 0.2 M Ammonium acetate, 0.1 M Tris pH 8.5, 25% w/v PEG 3,350, 277 K, VAPOR DIFFUSION, HANGING DROP |
