3LVY
Crystal Structure of Carboxymuconolactone Decarboxylase Family Protein SMU.961 from Streptococcus mutans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-03 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97921 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 97.996, 73.741, 183.708 |
| Unit cell angles | 90.00, 98.68, 90.00 |
Refinement procedure
| Resolution | 34.160 - 2.100 |
| R-factor | 0.187 |
| Rwork | 0.184 |
| R-free | 0.23600 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.238 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.160 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 75930 | |
| <I/σ(I)> | 12.6 | 2 |
| Completeness [%] | 99.5 | 98.5 |
| Redundancy | 3.6 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.1 M Hepes pH 7.5, 0.2 M Ammonium Sulphate, 25 % PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
