3LVK
Crystal Structure of E.coli IscS-TusA complex (form 2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-11 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9793 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 72.893, 131.407, 106.411 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.348 - 2.442 |
R-factor | 0.209 |
Rwork | 0.207 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1P3W and 1DCJ |
RMSD bond length | 0.007 |
RMSD bond angle | 1.010 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.6_289) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.540 |
High resolution limit [Å] | 2.442 | 5.280 | 2.442 |
Rmerge | 0.077 | 0.039 | 0.385 |
Number of reflections | 17507 | ||
<I/σ(I)> | 15.5 | ||
Completeness [%] | 91.3 | 97.4 | 58.3 |
Redundancy | 7.8 | 7.9 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 20% PEG 3350, 0.12M Magnisum Formate, vapor diffusion, sitting drop, temperature 295K |