3LVG
Crystal structure of a clathrin heavy chain and clathrin light chain complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Detector technology | CCD |
Collection date | 2002-03-03 |
Wavelength(s) | 1.017 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 228.560, 228.560, 710.320 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 100.000 - 7.940 |
Rwork | 0.419 |
R-free | 0.42500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.024 |
RMSD bond angle | 2.853 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 250.000 | 8.550 |
High resolution limit [Å] | 7.900 | 7.900 |
Number of reflections | 10700 | |
<I/σ(I)> | 20 | 2.8 |
Redundancy | 9 | 9.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 200mM citrate, 16-22% glycerol, 2% trifluoroethanol, vapor diffusion, hanging drop, temperature 298K |