3LSS
Trypanosoma brucei seryl-tRNA synthetase in complex with ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-19 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 175.652, 175.652, 248.518 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.570 - 1.950 |
| R-factor | 0.169 |
| Rwork | 0.168 |
| R-free | 0.19500 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3lsq |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.331 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.090 | 0.808 |
| Number of reflections | 106754 | |
| <I/σ(I)> | 9.1 | 1.5 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 8 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | sitting drop vapor diffusion | 7 | 298 | 1 ul 11 mg/ml protein in SGPP buffer mixed with 0.5 ul 2.4 M sodium malonate; crystal then soaked in 3.4 M sodium malonate (pH 7.0) supplemented with 10 mM ATP for 25 minutes before freezing., sitting drop vapor diffusion, temperature 298K |
