3LSR

Crystal structure of DesT in complex with duplex DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-11
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	I 41
Unit cell lengths	79.358, 79.358, 145.458
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.380 - 2.550
R-factor	0.22926
Rwork	0.228
R-free	0.26477
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lsp
RMSD bond length	0.010
RMSD bond angle	1.423
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.500
Rmerge	0.049
Number of reflections	14862
<I/σ(I)>	40.4
Completeness [%]	96.1
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	grown under mineral oil	7	291	ML- 0.1M Mes pH7.0, 9% PEG 20K. Protein sample- 0.3 mM protein, 0.3 mM oligo, 0.3 mM 18:1delta9-CoA. Drop- 2ul ML + 2 ul protein + 0.5 uL 1M ammonium sulfate., grown under mineral oil, temperature 291K