3LSP

Crystal Structure of DesT bound to desCB promoter and oleoyl-CoA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2007-10-08
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	I 41
Unit cell lengths	78.385, 78.385, 147.988
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.730 - 2.660
R-factor	0.22515
Rwork	0.223
R-free	0.26136
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.283
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.650
Rmerge	0.072
Number of reflections	12753
<I/σ(I)>	28.4
Completeness [%]	98.9
Redundancy	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	ML- 0.1 M Mes pH7.0, 9%PEG 20K. Protein sample- 0.3 mM protein, 0.3 mM oligo, 0.3 mM 18:1delta9-CoA. Drop- 2ul ML + 2ul protein + 0.5 ul 1M ammonium sulfate., VAPOR DIFFUSION, SITTING DROP, temperature 291K