3LSK
Pyranose 2-oxidase T169S acetate complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-13 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 100.256, 102.454, 136.716 |
| Unit cell angles | 90.00, 91.13, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.950 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.25600 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.004 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | FFT |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.700 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 198647 | |
| <I/σ(I)> | 6.2 | 1.8 |
| Completeness [%] | 98.8 | 99.3 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 298 | 0.1M sodium acetate, 0.05M MgCl2, 6% (w/v)% monomethylether PEG 2000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
