3LSE
N-Domain of human adhesion/growth-regulatory galectin-9 in complex with lactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210r |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 32.387, 32.387, 232.208 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.030 - 2.690 |
| R-factor | 0.22918 |
| Rwork | 0.223 |
| R-free | 0.35202 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a3k |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.938 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.030 | 2.750 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rmerge | 0.072 | 0.388 |
| Number of reflections | 3930 | |
| <I/σ(I)> | 21.1 | 5.1 |
| Completeness [%] | 96.7 | 100 |
| Redundancy | 9.4 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 30 % PEG 2000, 0.1 M Hepes (pH 7.0), VAPOR DIFFUSION, HANGING DROP, temperature 298K |
