3LSD
N-Domain of human adhesion/growth-regulatory galectin-9
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM16 |
Synchrotron site | ESRF |
Beamline | BM16 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 210r |
Spacegroup name | P 31 1 2 |
Unit cell lengths | 37.813, 37.813, 153.500 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.030 - 2.030 |
R-factor | 0.194 |
Rwork | 0.191 |
R-free | 0.26000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1a3k |
RMSD bond length | 0.020 |
RMSD bond angle | 1.817 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.030 | 2.120 |
High resolution limit [Å] | 2.010 | 2.010 |
Rmerge | 0.074 | 0.215 |
Number of reflections | 8444 | |
<I/σ(I)> | 17.8 | 6.7 |
Completeness [%] | 95.6 | 77.5 |
Redundancy | 6.6 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 298 | 26 % (w/v) PEG 4000, 0.25 M Na acetate, 0.1 M Tris (pH 8.5), VAPOR DIFFUSION, HANGING DROP, temperature 298K |