3LRY
Crystal structure of synthetic HIV-1 capsid C-terminal domain (CCA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-02-25 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 82.907, 58.932, 58.732 |
| Unit cell angles | 90.00, 133.45, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.980 |
| R-factor | 0.216 |
| Rwork | 0.213 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a43 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.593 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.636 | 2.010 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.065 | 0.578 |
| Number of reflections | 13819 | |
| <I/σ(I)> | 14.5 | 2 |
| Completeness [%] | 96.0 | 95.6 |
| Redundancy | 3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1 M HEPES, 0.8 M potassium phosphate monohydrate, 0.8M potassium phosphate monobasic, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
