3LRR

Crystal structure of human RIG-I CTD bound to a 12 bp AU rich 5' ppp dsRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	174
Detector technology	IMAGE PLATE
Collection date	2009-09-01
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.542
Spacegroup name	P 65
Unit cell lengths	83.010, 83.010, 111.220
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.190 - 2.150
R-factor	0.187
Rwork	0.187
R-free	0.22800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Free RIG-I CTD pdb ID. 2QFB chain A
RMSD bond length	0.009
RMSD bond angle	1.540
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.230
High resolution limit [Å]	2.150	2.150
Rmerge	0.071	0.457
Number of reflections	22092
<I/σ(I)>	36	4.4
Completeness [%]	93.5	90.4
Redundancy	6.5	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	PEG 3,350 (15%) plus ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K