3LRH
Structure of anti-huntingtin VL domain in complex with huntingtin peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-11-03 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.090, 89.660, 95.000 |
| Unit cell angles | 90.00, 99.45, 90.00 |
Refinement procedure
| Resolution | 19.860 - 2.600 |
| R-factor | 0.21885 |
| Rwork | 0.216 |
| R-free | 0.27804 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lrg |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.828 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.114 | 0.318 |
| Number of reflections | 24090 | |
| <I/σ(I)> | 9.8 | 4.1 |
| Completeness [%] | 88.7 | 92 |
| Redundancy | 2.8 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.1 | 293 | 10 % PEG600, 0.1 mM potassium phosphate dibasic/citric acid, pH 4.1, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
