3LR5
Periplasmic domain of the risS sensor protein from Burkholderia pseudomallei, iodide phased at neutral pH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-04 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.040, 76.010, 88.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.610 - 2.300 |
| R-factor | 0.206 |
| Rwork | 0.203 |
| R-free | 0.25200 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.504 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.610 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.092 | 0.410 |
| Number of reflections | 13048 | 947 |
| <I/σ(I)> | 15.79 | 4.6 |
| Completeness [%] | 98.9 | 99.8 |
| Redundancy | 6.69 | 6.25 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.1 M BisTris Propane pH 6.5, 0.2 M NaI, 25% PEG 3350, crystal tracking ID 203088f3, affinity tag not removed prior to crystallization, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
