3LR1
The crystal structure of the tungstate ABC transporter from Geobacter sulfurreducens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 69.736, 70.386, 91.161 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.540 - 1.800 |
| R-factor | 0.18516 |
| Rwork | 0.181 |
| R-free | 0.26527 |
| Structure solution method | SAD |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.954 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.540 | 1.847 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.091 | 0.817 |
| Number of reflections | 19739 | |
| <I/σ(I)> | 23.83 | 1.88 |
| Completeness [%] | 98.3 | 96.72 |
| Redundancy | 9 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.2M ammonium sulfate, 0.1M bis-tris, 25% PEG3350, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
