3LQV

Branch Recognition by SF3b14

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-01-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.11586
Spacegroup name	C 2 2 21
Unit cell lengths	101.848, 112.702, 82.163
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.470 - 2.380
R-factor	0.216
Rwork	0.214
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.551
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	REFMAC (5.2.0005)
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.490
High resolution limit [Å]	2.380	5.170	2.380
Rmerge	0.055	0.024	0.301
Number of reflections	18348
<I/σ(I)>	15.3
Completeness [%]	95.9	99.6	84
Redundancy	3.8	3.9	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6	298	PEG3350, 0.2M NaCl, 20mM Adenine, pH 6, vapor diffusion, temperature 298K