3LQQ
Structure of the CED-4 Apoptosome
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97623 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 181.640, 181.640, 203.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.664 - 3.534 |
| R-factor | 0.2768 |
| Rwork | 0.275 |
| R-free | 0.31120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a5y |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.437 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.664 | 3.680 |
| High resolution limit [Å] | 3.534 | 3.530 |
| Rmerge | 0.089 | 0.508 |
| Number of reflections | 20874 | |
| <I/σ(I)> | 14.6 | 1.78 |
| Completeness [%] | 98.8 | 94.1 |
| Redundancy | 3.9 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.1M Tris-HCl, pH 8.5, 0.7M sodium potassium tartrate, 200mM Cymal-1 (Anatrace), VAPOR DIFFUSION, HANGING DROP, temperature 291K |
