3LQA

Crystal structure of clade C gp120 in complex with sCD4 and 21c Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-23
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.953
Spacegroup name	C 2 2 21
Unit cell lengths	93.730, 187.930, 151.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	84.000 - 3.400
Rwork	0.234
R-free	0.32200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.024
RMSD bond angle	3.475
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	84.000	3.580
High resolution limit [Å]	3.400	3.400
Number of reflections	14975
<I/σ(I)>		1.9
Completeness [%]	79.0
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	20% (w/v) PEG monomethyl ether 2000, 5% (v/v) PEG 200, 0.2 M Trimethylamine N-oxide, 0.1 M Tris-HCl pH 8.5 , VAPOR DIFFUSION, SITTING DROP, temperature 293K