3LPN
Crystal structure of the phosphoribosylpyrophosphate (PRPP) synthetase from Thermoplasma volcanium in complex with an ATP analog (AMPCPP).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.576, 141.162, 50.039 |
| Unit cell angles | 90.00, 95.97, 90.00 |
Refinement procedure
| Resolution | 29.174 - 1.800 |
| R-factor | 0.1758 |
| Rwork | 0.174 |
| R-free | 0.21590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u9y |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.030 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | PHENIX (1.4_4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.114 | 0.594 |
| Number of reflections | 49153 | |
| <I/σ(I)> | 11.7 | 2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 2M Ammonium sulfate, 0.1M citrate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
