3LPL
E. coli pyruvate dehydrogenase complex E1 component E571A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2004-08-11 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.820, 142.220, 82.520 |
| Unit cell angles | 90.00, 102.36, 90.00 |
Refinement procedure
| Resolution | 41.720 - 2.100 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.23800 |
| Structure solution method | DIFFERENCE FOURIER |
| Starting model (for MR) | 2iea |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.720 | 2.230 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.090 | 0.287 |
| Number of reflections | 101325 | |
| <I/σ(I)> | 22.41 | 6.3 |
| Completeness [%] | 95.0 | 88.2 |
| Redundancy | 7.5 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | PEG2000 MONOMETHYL ETHER, PROPANOL, SODIUM AZIDE, HEPES BUFFER, MAGNESIUM CHLORIDE, THIAMIN DIPHOSPHATE, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
