3LOQ

The crystal structure of a universal stress protein from Archaeoglobus fulgidus DSM 4304

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-19
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97910
Spacegroup name	P 21 21 21
Unit cell lengths	72.308, 89.169, 137.784
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.240 - 2.320
R-factor	0.1809
Rwork	0.179
R-free	0.22480
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.243
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	SHELXD
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.300	2.370
High resolution limit [Å]	2.320	2.330
Rmerge	0.079	0.762
Number of reflections	38893
<I/σ(I)>	24.7	2.1
Completeness [%]	99.4	99.6
Redundancy	4.7	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	277	0.1M Sodium Acetate trihydrate 3.0M NaCl, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K