3LNP

Crystal Structure of Amidohydrolase family Protein OLEI01672_1_465 from Oleispira antarctica

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-11
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.97924
Spacegroup name	P 32 2 1
Unit cell lengths	77.902, 77.902, 143.754
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.246 - 2.100
R-factor	0.151
Rwork	0.149
R-free	0.18400
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.056
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.250	2.140
High resolution limit [Å]	2.100	2.100
Number of reflections	30246
<I/σ(I)>	9.6	4
Completeness [%]	99.9	100
Redundancy	11.8	10.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	9 % PEG8000, 0.2 M Calcium Acetate, 0.1 M sodium cacodylate pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K