3LNO
Crystal Structure of Domain of Unknown Function DUF59 from Bacillus anthracis
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97924 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 57.458, 78.556, 174.116 |
| Unit cell angles | 90.00, 99.30, 90.00 |
Refinement procedure
| Resolution | 45.940 - 2.100 |
| R-factor | 0.168 |
| Rwork | 0.165 |
| R-free | 0.21800 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.335 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.940 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 42911 | |
| <I/σ(I)> | 12.5 | 3.5 |
| Completeness [%] | 96.3 | 92.9 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 289 | 0.1 M Acetate, 20% (v/v) 1,4-butanediol, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
