3LNF
Crystal structure of E-cadherin EC12 K14EW2A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-16 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97897 |
Spacegroup name | P 1 |
Unit cell lengths | 45.107, 47.383, 69.897 |
Unit cell angles | 70.58, 88.13, 75.50 |
Refinement procedure
Resolution | 28.635 - 2.500 |
R-factor | 0.1872 |
Rwork | 0.184 |
R-free | 0.24080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1edh |
RMSD bond length | 0.011 |
RMSD bond angle | 1.337 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.500 |
Rmerge | 0.111 |
Number of reflections | 17683 |
<I/σ(I)> | 8.2 |
Completeness [%] | 98.6 |
Redundancy | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 1.2M ammonium sulfate, 0.1M Tris-Cl pH 8.5, 15% (v/v) glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |