3LNC

Crystal structure of guanylate kinase from Anaplasma phagocytophilum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-01-12
Detector	ADSC QUANTUM 210r
Spacegroup name	P 21 21 21
Unit cell lengths	64.530, 76.580, 103.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.950
R-factor	0.2104
Rwork	0.209
R-free	0.24380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2anb modified with CCP4 program CHAINSAW
RMSD bond length	0.014
RMSD bond angle	1.419
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.000
High resolution limit [Å]	1.950	1.950
Rmerge	0.067	0.485
Number of reflections	38137
<I/σ(I)>	19.34	4.4
Completeness [%]	100.0	100
Redundancy	8	8.14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	290	EBS JCSG+ SCREEN, B6: 100MM PHOSPHATE/CITRATE, 40% ETHANOL, 5% PEG 1000. ANPHA.01463.A AT 52MG/ML, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 290K