3LN3

Crystal structure of Putative reductase (NP_038806.2) from MUS MUSCULUS at 1.18 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-05
Detector	MARMOSAIC 325 mm CCD
Spacegroup name	C 1 2 1
Unit cell lengths	157.506, 47.206, 47.100
Unit cell angles	90.00, 94.98, 90.00

Refinement procedure

Resolution	28.895 - 1.180
R-factor	0.132
Rwork	0.131
R-free	0.15600
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.827
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.5.0053)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.895	28.895	1.220
High resolution limit [Å]	1.180	2.540	1.180
Rmerge	0.064	0.037	0.457
Number of reflections	110769	21504	14203
<I/σ(I)>	6.23	16.6	1.5
Completeness [%]	95.0	96.2	67.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	2.4000M ammonium sulfate, 0.1M TRIS pH 8.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K