3LMO
Crystal Structure of specialized acyl carrier protein (RPA2022) from Rhodopseudomonas palustris, Northeast Structural Genomics Consortium Target RpR324
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-17 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.97852 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 62.730, 62.730, 60.125 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.430 - 2.000 |
R-factor | 0.217 |
Rwork | 0.216 |
R-free | 0.25400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.000 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.2 & XtalView) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.033 | 0.086 |
Number of reflections | 16068 | |
<I/σ(I)> | 51.2 | 14.6 |
Completeness [%] | 89.8 | 67.4 |
Redundancy | 6.9 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microbatch, under oil | 4.2 | 277 | Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5), Reservoir solution: 0.1M sodium Citrate (pH 4.2), 12% PEG 20k, and 0.1M lithium sulfate, Microbatch, under oil, temperature 277K |