3LLT
Crystal structure of PF14_0431, kinase domain.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | .97949 |
| Spacegroup name | P 65 |
| Unit cell lengths | 140.081, 140.081, 52.418 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.500 |
| R-factor | 0.225 |
| Rwork | 0.223 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z57 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.008 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.114 | 0.449 |
| Number of reflections | 20417 | |
| <I/σ(I)> | 9.6 | 2.19 |
| Completeness [%] | 99.3 | 94.6 |
| Redundancy | 5.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 1.85 M NH4SO4 0.2 M NaAcet 0.1 M Na Caodylate pH 5.6 5 mM AMPPNP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
