3LK4
Crystal structure of CapZ bound to the uncapping motif from CD2AP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 105 |
Detector technology | CCD |
Collection date | 2008-11-14 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 1 |
Unit cell lengths | 59.728, 142.341, 192.999 |
Unit cell angles | 90.00, 89.99, 90.00 |
Refinement procedure
Resolution | 19.984 - 1.990 |
R-factor | 0.2277 |
Rwork | 0.225 |
R-free | 0.27220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lk2 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.020 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.100 |
High resolution limit [Å] | 1.990 | 2.000 |
Rmerge | 0.054 | 0.418 |
Number of reflections | 358158 | |
<I/σ(I)> | 24.7 | 3.3 |
Completeness [%] | 82.6 | 76.8 |
Redundancy | 3.2 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 30% PEG 400, 0.1M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |