3LJQ

Crystal Structure of the Glycosylasparaginase T152C apo-precursor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Spacegroup name	P 1
Unit cell lengths	46.061, 52.533, 61.933
Unit cell angles	81.47, 90.21, 104.93

Refinement procedure

Resolution	31.740 - 1.900
R-factor	0.154
Rwork	0.154
R-free	0.19700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	9gac
RMSD bond length	0.013
RMSD bond angle	1.620
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.740	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.029	0.059
Number of reflections	42606
<I/σ(I)>	26.7	13.3
Completeness [%]	97.2
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5	277	15% PEG 3350, 100 mM HEPES pH 7.5, 0.1% sodium azide, VAPOR DIFFUSION, HANGING DROP, temperature 277K