3LJF
The X-ray structure of iron superoxide dismutase from Pseudoalteromonas haloplanktis (crystal form II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-26 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.476, 103.785, 90.251 |
| Unit cell angles | 90.00, 103.81, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.192 |
| Rwork | 0.192 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1isa |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.100 |
| Rmerge | 0.092 |
| Number of reflections | 51958 |
| Completeness [%] | 99.4 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | Crystals of protein were obtained using 2.3-2.5M ammonium sulphate, 10 mM FeCl3, 100 mM HEPES pH 7.0, 3% (v/v) isopropanol, VAPOR DIFFUSION, HANGING DROP |
