3LJE

The X-ray structure of zebrafish RNase5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-27
Detector	RIGAKU SATURN 944
Spacegroup name	I 41 3 2
Unit cell lengths	126.489, 126.489, 126.489
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.810 - 1.800
R-factor	0.169
Rwork	0.169
R-free	0.20000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ljd
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.800
Rmerge	0.095
Number of reflections	16327
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.5	293	32% MPEG 2K, 0.2M ammonium sulfate, 0.1M sodium acetate , pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K