3LJB
Structural basis of oligomerisation in the MxA stalk
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-08-07 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.91841 |
Spacegroup name | P 1 |
Unit cell lengths | 50.147, 57.366, 57.491 |
Unit cell angles | 65.82, 88.47, 74.87 |
Refinement procedure
Resolution | 19.950 - 2.400 |
R-factor | 0.19634 |
Rwork | 0.193 |
R-free | 0.25681 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Initial model derived from a low-resolution SeMet data set |
RMSD bond length | 0.011 |
RMSD bond angle | 1.152 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.460 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.103 | 0.473 |
Number of reflections | 21393 | |
<I/σ(I)> | 14.91 | 3.76 |
Completeness [%] | 97.1 | 96.7 |
Redundancy | 3.52 | 3.54 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 300 | 5% PEG3350, 100 mM MES (pH 6.8), 100 mM MgCl2 and 0.01 mM Hexammine cobalt (III) chloride, VAPOR DIFFUSION, SITTING DROP, temperature 300K |